Ca2+influx via the L‐type Ca2+channel during tail current and above current reversal potential in ferret ventricular myocytes
نویسندگان
چکیده
منابع مشابه
Cholinergic modulation of the basal L-type calcium current in ferret right ventricular myocytes.
The effects of the cholinergic muscarinic agonist carbachol (CCh) on the basal L-type calcium current, I(Ca,L), in ferret right ventricular (RV) myocytes were studied using whole cell patch clamp. CCh produced two major effects : (i) in all myocytes, extracellular application of CCh inhibited I(Ca,L) in a reversible concentration-dependent manner; and (ii) in many (but not all) myocytes, upon w...
متن کاملKinetic analysis of the L-type calcium current in enzymatically dissociated ferret ventricular myocytes.
The L-type calcium current (ICa-L) was studied in single ferret ventricular myocytes using whole-cell recording with single patch pipettes. Voltage-clamp experiments were performed at room temperature with internal and external Na(+)- and K(+)-free Tyrode solutions in order to isolate ICa-L. For depolarizing steps eliciting small ICa-L the decay of the current is best described by one exponenti...
متن کاملBackground potassium current active during the plateau of the action potential in guinea pig ventricular myocytes.
Background outward K+ currents in guinea pig ventricular myocytes were characterized over a broad range of membrane potentials, including those corresponding to the plateau of the action potential. The background current that is blocked by 1 mM Ba2+ (IK,p) activates within 5 msec at positive potentials, does not inactivate, and deactivates very rapidly on repolarization. IK,p is insensitive to ...
متن کاملProfile of L-type Ca(2+) current and Na(+)/Ca(2+) exchange current during cardiac action potential in ventricular myocytes.
OBJECTIVE The L-type Ca(2+) current (I(Ca,L)) and the Na(+)/Ca(2+) exchange current (I(NCX)) are major inward currents that shape the cardiac action potential (AP). Previously, the profile of these currents during the AP was determined from voltage-clamp experiments that used Ca(2+) buffer. In this study, we aimed to obtain direct experimental measurement of these currents during cardiac AP wit...
متن کاملChloride Current in Guinea Pig Ventricular Myocytes
Cardiac ventricular myocytes from several species, including the guinea pig, possess a cAMP-dependent protein kinase A (PKA)-activated Clchannel. In the present study, the properties of a protein kinase C (PKC)-activated Clcurrent were studied in isolated guinea pig ventricular myocytes using the whole-cell arrangement of the patch-clamp technique. Intracellular dialysis of ventricular cells wi...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 2000
ISSN: 0022-3751,1469-7793
DOI: 10.1111/j.1469-7793.2000.t01-2-00057.x